![]() ![]() ![]() The bed height of a gravity-settled bed differs from the bed height of a bed settled at low flow (consolidated). Packing MabSelect PrismA™ resin in AxiChrom™ columns Definitions We provide verified chromatography resin packing methods for these columns. AxiChrom™ columns provide you with excellent packing control. We also provide a short guideline for larger columns.įig 1. The chromatography column packing methods we describe here apply to bed heights of up to 30 cm in columns of up to 1000 mm i.d. Intelligent Packing enables straightforward operation and high packing success rates. The columns feature Intelligent packing with preprogrammed methods that support all column sizes. The columns are designed to give you scalable and predictable results over the entire range of scales by enabling a uniform plug flow through the bed, irrespective of column size. Mechanical axial compression enables accurate and reproducible control of the packing, even with large-diameter columns.ĪxiChrom™ columns are available in many different configurations and materials. AxiChrom™ columnsĪxiChrom™ columns are low-pressure, mechanical axial compression chromatography columns designed for process development and biopharmaceutical manufacturing environments (Fig 1). The resin offers highly concentrated elution pools, which gives you operating flexibility and small unit operation. To 1.0 M sodium hydroxide (NaOH) for cleaning in place (CIP). The protein A-derived ligand lets you use 0.5 The high-ligand stability of MabSelect PrismA™ reduces ligand leakage and offers exceptional alkali-tolerance. The combination of a high binding capacity and a high-flow base matrix gives excellent productivity.įlow velocity for MabSelect PrismA™ is at least 300 cm/h in an AxiChrom™ 300 column with 20 cm bed height at 20☌ using process buffers with the same viscosity as water and pressure over bed < 2 bar. MabSelect PrismA™ is an affinity BioProcess™ resin for capturing monoclonal and bispecific antibodies (mAbs and bsAbs) as well as Fc-containing recombinant proteins. MabSelect PrismA™ chromatography resin characteristics The procedures we describe include robust and verified packing methods that will help you eliminate concerns and risks associated with poorly packed beds.Ĭlick here for more resources on process-scale column packing. We describe best practices for packing MabSelect PrismA™ resin in large-scale AxiChrom™ columns, BPG glass columns, and Chromaflow™ chromatography columns. Proper column packing ensures a stable bed that performs according to expectations over many processing cycles. A bed packed too loosely can further compress causing a liquid gap where mixing can occur.Įither instance will lead to costly process disruptions and loss of valuable product. A bed packed too densely might crack, which can lead to channeling and early breakthrough. Good column packing is essential for any chromatographic process and plays a key role in bioprocessing of biopharmaceuticals. ![]()
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